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Despite various merits, there are some demerits such as the cost of FRET reagents, selection of fluorophores, stability of functionalized nanoscale materials, low fluorescence resolution and low signal-to-noise ratio. Electrochemiluminescence (ECL) or electron generated chemiluminescence based biosensors are emerging and less explored categories of biosensors which are gaining increasing attention. These are based on the phenomenon in which the luminescent species absorb energy from the electrochemical reaction occurring near their surface. Then aptamer conjugated MXenes were attached to the modified electrode, followed by immersion in HAuCl4 for in situ synthesis of AuNPs. The MXenes acted both as stabilizers and reductants, and the in situ formed AuNP–MXene hybrid not only demonstrated highly efficient recognition of exosomes but also provided a large catalytic surface with AuNPs. A 1000 times lower detection limit (i.e. 30 particles per μL) than that of the conventional ELISA was achieved.

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